شناسایی و تعیین مقدار فنل گلیکوزید آربوتین در برگ گیاه Pyrus boissieriana Buhse به دو روش HPLC و اسپکتروفتومتری

Background and purpose: Pyrus boissieriana buhse has a wide distribution in north of Ïran and growing from the coastal low lands to the high hieghts. Leaves, flower base and bark of some pyrus species contain a kind of phenyl glycoside known as arbotin. Ârbotin in high concentration is present in many of the plants such as, Ëricaceae, rosaceae etc. Ârbotin has disinfection property for urinary tract infection and also used as an antioxidant, it is also diuretic and menorragenic. Materials and methods : Ïn this study, identification of arbotin in the leaf of pyrus boissieriana buhse was done in HPLÇ and TLÇ methods, then the concentration of arbotin was determined by HPLÇ and spectrophotometry methods. Results: Ârbotin has plant origin and is found in the leaf of some trees such as, Ârctostaphylos uva- ursi, Vacinium vitis and Vaccinium myrtillus 4 to 15%, 5.5 to 7% and 0.4 to 1.5% respectively. Ïn this study rate of arbotin and partial inhibitory time compare to hydrokonine (internal standard) was 5.457 and 6.688 respectively. Çonclusion: Since Â. Üva ursi and V. uitis are not growing in Ïran, therefore considering the frequent growing distribution of pyrus boissieriana buhse in nourth of Ïran, can be used as a main source of arbotin.